Regulatory

Part:BBa_K1980004:Design

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)


pCusC promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We found a version of the pCusC promoter in the registry here: (https://parts.igem.org/Part:BBa_I760005). This part had 11 uses but had no expression data associated with any of these. As the part is only 16 nucleotides long, we believe it is far too small and missing important binding sites for both CusR and RNA polymerase. We extended this part in the upstream direction.

Source

Original source is the E. coli genome. Ordered together with mKate2 from IDT and the promoter alone was obtained by PCR.

References

Yamamoto K, Ishihama A. (2005) “Transcriptional response of Escherichia coli to external copper.” Mol Microbiol. 2005 Apr;56(1):215-27.